Bio-pharmaceutical and pharmaceutical analysis. Coordinator: Bartolini

Studies on the modulation of bio-recognition phenomena, the structural alterations of pharmaceutically relevant proteins and the chirality of drugs by means of spectroscopic, spectrometric and chromatographic techniques. Development/validation of analytical methods for the quality control of drugs

Research themes

Subjects related to drug discovery and biomedical area

Studies on molecular bio-recognition phenomena and determination of the kinetic and thermodynamic binding parameters (kon, koff, KD) by surface plasmon resonance (SPR) optical biosensor, circular dichroism (CD) and fluorescence spectroscopies.

Characterization of structural alterations in proteins of pharmaceutical interest by liquid chromatography coupled to mass spectrometry (LC-MS) and CD analysis.

Development of analytical tools based on immobilized enzymes and proteins for online studies (inhibition, binding, tryptic digestion and N-deglycosylation). 

Areas of study:

Study of structural alterations of circulating serum albumin as marker in diseases characterized by high levels of oxidative stress and inflammation (cirrhosis and diabetes).

Screening of cholinesterase inhibitors and screening of inhibitors of b-amyloid and/or tau aggregation for the treatment of Alzheimer's disease. 

Investigations on the secondary structure of proteins by circular dichroism.

Subjects related to the pharmaceutical field

Development and validation of new HPLC methods for drug and nutraceutical (dietary supplements) quality control in new formulations or in commercially available products, containing active ingredients of synthetic and/or natural origin.

Lab members

Prof. Manuela Bartolinie-mail and web site

Dr. Marina Naldi (Senior researcher - RTDb) – e-mail and web site

Marta Nugnes (PhD student) - e-mail

Internships projects

Internships for students belonging to CTF, Biotechnology and Pharmaceutical Biotechnology.

The topics of recent theses (undergraduated students) are available on the group's component sites.

Main publications

A Tramarin, M Naldi, G Degani, L Lupu, P Wiegand, A Mazzolari, A Altomare, G Aldini, L Popolo, G Vistoli, M Przybylski, M Bartolini. Unveiling the molecular mechanisms underpinning biorecognition of early-glycated human serum albumin and receptor for advanced glycation end products. Analytical and Bioanalytical Chemistry, 2020, 412, 4245. DOI: 10.1007/s00216-020-02674-w

E Fabini, A Tramarin, M Bartolini. Combination of human acetylcholinesterase and serum albumin sensing surfaces as highly informative analytical tool for inhibitor screening. J Pharm Biomed Anal 2018, 155, 177. DOI: 10.1016/j.jpba.2018.03.060

M Naldi, U Černigoj, A Štrancar, M Bartolini. Towards automation in protein digestion: Development of a monolithic trypsin immobilized reactor for highly efficient on-line digestion and analysis. Talanta 2017, 167, 143. DOI: 10.1016/j.talanta.2017.02.016

X Zha, D Lamba, L Zhang, Y Lou, C Xu, D Kang, L Chen, Y Xu, L Zhang, A De Simone,  S Samez,  A Pesaresi,  J Stojan,  MG Lopez,  J Egea,  V Andrisano,  M Bartolini. Novel tacrine-benzofuran hybrids as potent multitarget-directed ligands for the treatment of Alzheimer’s disease: design, synthesis, biological evaluation, and X-ray crystallography. J Med Chem 2016, 59, 114. DOI:10.1021/acs.jmedchem.5b01119

M Bartolini, C Bertucci, ML Bolognesi, A Cavalli, C Melchiorre, V Andrisano. Insight into the kinetic of amyloid b(1–42) peptide self-aggregation: elucidation of inhibitors’ mechanism of action. ChemBioChem 2007, 8, 2152. DOI: 10.1002/cbic.200700427