Figure 1: 3D printing slicer view of microfluidics schemes useful for CE-MS by electron ionization, and examples of electropherograms by CE-LEDIF of derivatized amino acids.
Research themes
1) Separation techniques at analytical and microanalytical scale (CE, UHPLC, GC-MS) addressed to bioactive compounds determination.
Chromatographic and electrokinetics methods are developed and applied to the determination of bioactive compounds in complex samples e.g., biological, natural extracts and environmental samples. The development involves the use and implementation of sample preparation techniques as solid-phase microextraction (SPME), solid-phase extraction (SPE) also by using sorbents based on molecular imprinting (MIP). The optimization and validation of the analytical methods is carried out by chemometric approaches as Design of Experiments (DoE) and Quality by Design (QbD).
2) Implementation of detection systems for electrokinetics separation at microanalytical scale (CE-LEDIF, CE-EIMS).
Laser-induced fluorescence (LIF) and light-emitting diode-induced fluorescence (LEDIF) are among the most sensitive detection approaches in CE. Only few compounds possess the native fluorescence to be directly detected by LIF and LEDIF, thus derivatization is often necessary to introduce into the target compounds the molecular moieties able to fluoresce under laser o LED excitation. The research is addressed to the development of derivatization techniques using novel fluorescent tags addressed to a variety of molecules of biological, pharmaceutical, and environmental interest.
An original hyphenation mode for combining CE to mass spectrometry (MS) is developing by the direct introduction of the CE effluent into the electron impact (EI) source by means of home-made 3D-printed interfaces.