Neural Repair - Coordinator: Laura Calzà

Research themes

1. Experimental neurological diseases and injuries. 2. Rare diseases with a prevalent neurological phenotype. 3. Complex cell systems based on stem cells for testing innovative therapeutic solutions.

1.  Experimental neurological diseases and injuries. A. Risk cofactors in Azheimer's disease. The sporadic form of Alzheimer's dementia is recognized as a multifactorial disease with a very long presymptomatic phase. Our group studies physiological and pathological and  iatrogenic factors  which, in the preclinical phase of the disease, can modify its natural history, both by accelerating it and slowing it down, starting from the constitution and stability of the cognitive reserve. B. Neonatal encephalopathy: white matter protection. Hypoxic-ischemic suffering during childbirth, as well as severely preterm birth and low birth weight are factors that can lead to severe sensory-sensory, locomotor and cognitive disability. Our group studies the effect of hypoxia on stem cells and endogenous precursors which in this phase of development guarantee the completion of neurogenesis and the start of myelination, and possible therapeutic strategies to limit the damage. C. Spinal cord injury. Contusive spinal cord injury causes severe motor and sensory disabilities, and is considered an "orphan condition" due to the absence of therapies other than surgical stabilization of the vertebral segments and rehabilitation. Our group develops and tests in animal models therapies based on drugs, biomaterials, physical energies, to obtain a reduction of "secondary degeneration", the control of scar formation, promote remyelination and promote the "pruning" of residual axons, favoring therefore a better functional outcome.

2. Rare diseases with a prevalent neurological phenotype. Our group, starting from its experience in the analysis of the neurological phenotype from birth to adulthood and in collaboration with national and international research laboratories, is characterizing new models of transgenic mice (KO and KI) and testing gene, enzyme replacement and dietary therapies for numerous genetic diseases (Alport, Pompe, AGU, CDKL5, Laman, GRIN2A, etc.). In parallel, it studies patient-derived cell systems carrying the same mutations (fibroblasts and iPSC).

3. Complex cell systems based on stem cells for testing innovative therapeutic solutions. Starting from a consolidated experience in the isolation and in vitro study of embryonic and neural stem cells, we develop complex and three-dimensional cell culture systems, including biomaterials and microfluidics systems (organ-on-chip). These systems are mainly used for screening the biological effects of drugs and chemicals, and analyzed using cell-based high content analysis technologies.

Lab members

Laura Calzà, Full Professor

Corinne Quadalti, Junior Assistant professor

Marco Cristani, PhD student (in co-tutoring with Prof. Patrizia Romualdi)

Job openings or internship projects

There are currently no positions available.

Main publications

• Flagelli A, Candini O, Frabetti S, Dominici M, Giardino L, Calza L, Baldassarro VA. A novel three-dimensional culture device favors a myelinating morphology of neural stem cell-derived oligodendrocytes. Frontiers Cell and Developmental Biology, 2021, Oct 1;9:759982 https://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2021.759982/full
• Baldassarro VA, Perut F, Cescatti M, Pinto V, Fazio N, Alastra G, Parziale V, Bassotti A, Fernandez M, Giardino L, Baldini N, Calzà L, Intra-individual variability in the promyelinating properties of conditioned culture medium obtained from human adipose mesenchymal stromal cells. Stem Cell Research & Therapy, 2023, 14:128-146 https://stemcellres.biomedcentral.com/articles/10.1186/s13287-023-03344-1
• Ferroni L, D’Amora U, Gardin C, Leo S, Dalla Paola L, Tremoli E, Giuliani A, Calzà L, Ronca A, Ambrosio L, Zavan B, Stem cell-derived small extracellular vesicles embedded into methacrylated hyaluronic acid wound dressings accelerate wound repair in a pressure model of diabetic ulcer. Journal of Nanobiotechnology, 2023, 21:469-485 https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-023-02202-9
• Noera G, Bertolini A, Calzà L, Gori M, Pitino A, D’Arrigo G, Egan CG, Tripepi G, Effect of Early Administration of Targeting Melanocortin Receptor on Mortality and Host Response in Critically Ill Patients Requiring Rescue Surgery: A Sensitivity Analysis of The STOPSHOCK Phase 3 Randomized Controlled Trial. Military Medical Research, 2024, 11:56 https://mmrjournal.biomedcentral.com/articles/10.1186/s40779-024-00555-2
• Sannia M, Zanella L, Lorenzini L, Amedei, Moretti M, Cescatti M, Baldassarro AV, Quadalti C, Clavenzani P, DeGiorgio R, Calzà L, Giardino L, Experimental colitis in young Tg2576 mice accelerates the onset of an Alzheimer's-like clinical phenotype. Alz Res & Ther, 2024, 16:116-, 135 https://alzres.biomedcentral.com/articles/10.1186/s13195-024-01471-2