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Department of Pharmacy and Biotechnology - FABIT

Enzymology. Coordinator: Hochkoeppler

At present, we are engaged in 3 main research projects, namely: i) the characterization of the HoLaMa DNA polymerase, a replicase constructed in our laboratory; ii) the determination of the catalytic mechanism of Mycobacterium tuberculosis tyrosine phosphatase; iii) the investigation of allosteric transitions in rabbit lactate dehydrogenase.

Areas: Industrial, plant and environmental biotechnologies

Research themes

At present, we are engaged in 3 main research projects, namely: i) the characterization of the HoLaMa DNA polymerase, a replicase constructed in our laboratory; ii) the determination of the catalytic mechanism of Mycobacterium tuberculosis tyrosine phosphatase; iii) the investigation of allosteric transitions in rabbit lactate dehydrogenase.

Lab Members

Alejandro Hochkoeppler, Associate Professor

Alessandra Stefan, Senior Assistant Professor

Antonio Gonzalez Vara y Rodriguez, Senior Assistant Professor

Internship projects

3

Main publications

SINGH D., GAWEL D., ITSKO M., HOCHKOEPPLER A., KRAHN J.M., LONDON R.E., SCHAAPER R.M., 2015, Structure of Escherichia coli dGTP triphosphohydrolase: a hexameric enzyme with DNA effector molecules, Journal of Biological Chemistry, 290: 10418-10429.

MARTINA C.E., LAPENTA F., MONTON SILVA A., HOCHKOEPPLER A., 2015, Holama: a Klenow sub-fragment lacking the 3’-5’ exonuclease domain, Archives of Biochemistry and Biophysics, 575: 46-53.

MONTON SILVA A., LAPENTA F., STEFAN A., DAL PIAZ F., CECCARELLI A., PERRONE A., HOCHKOEPPLER A., 2015, Simultaneous ternary extension of DNA catalyzed by a trimeric replicase assembled in vivo, Biochemical and Biophysical Research Communications, 462: 14-20.

SINGH D., SCHAAPER R.M., HOCHKOEPPLER A., 2016, A continuous spectrophotometric enzyme-coupled assay for deoxynucleoside triphosphate triphosphohydrolases, Analytical Biochemistry, 496: 43-49.

FIJEN C., MONTON SILVA A., HOCHKOEPPLER A., HOHLBEIN J., 2017, A single-molecule FRET sensor for monitoring DNA synthesis in real time, Physical Chemystry Chemical Physics, 19: 4222-4230.

Contacts